Description
Aviva Systems Biology MTR ELISA Kit (Human) (OKEH02417) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human MTR antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MTR and incubated. The MTR found in the sample or standards competes with the biotinylated MTR for limited binding sites on the immobilized anti-Human MTR antibody. Excess unbound biotinylated MTR and sample or standard MTR is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MTR captured in the well and inversely proportional to the amount of MTR which was contained in the sample or standard